plasmid encoding vsvg Search Results


yfp-vsv-g tso45 mutant construct  (Genentech inc)
90
Genentech inc yfp-vsv-g tso45 mutant construct
Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G <t>tsO45</t> released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.
Yfp Vsv G Tso45 Mutant Construct, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/yfp-vsv-g tso45 mutant construct/product/Genentech inc
Average 90 stars, based on 1 article reviews
yfp-vsv-g tso45 mutant construct - by Bioz Stars, 2026-05
90/100 stars
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plasmids encoding viral packaging proteins vsvg and hit-60  (Promega)
90
Promega plasmids encoding viral packaging proteins vsvg and hit-60
Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G <t>tsO45</t> released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.
Plasmids Encoding Viral Packaging Proteins Vsvg And Hit 60, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmids encoding viral packaging proteins vsvg and hit-60/product/Promega
Average 90 stars, based on 1 article reviews
plasmids encoding viral packaging proteins vsvg and hit-60 - by Bioz Stars, 2026-05
90/100 stars
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plasmid encoding the vesicular stomatitis glycoprotein (vsvg)-tagged n-domain of ezrin (aa 1–309)  (Institut Curie)
90
Institut Curie plasmid encoding the vesicular stomatitis glycoprotein (vsvg)-tagged n-domain of ezrin (aa 1–309)
Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G <t>tsO45</t> released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.
Plasmid Encoding The Vesicular Stomatitis Glycoprotein (Vsvg) Tagged N Domain Of Ezrin (Aa 1–309), supplied by Institut Curie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid encoding the vesicular stomatitis glycoprotein (vsvg)-tagged n-domain of ezrin (aa 1–309)/product/Institut Curie
Average 90 stars, based on 1 article reviews
plasmid encoding the vesicular stomatitis glycoprotein (vsvg)-tagged n-domain of ezrin (aa 1–309) - by Bioz Stars, 2026-05
90/100 stars
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expression plasmids encoding mutant cdk4, cyclin d1, csii-cmv-htert, pcmv-vsvg-rsv, pcag-hivgp, and csii-cmv-egfp  (BioResource International Inc)
90
BioResource International Inc expression plasmids encoding mutant cdk4, cyclin d1, csii-cmv-htert, pcmv-vsvg-rsv, pcag-hivgp, and csii-cmv-egfp
Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G <t>tsO45</t> released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.
Expression Plasmids Encoding Mutant Cdk4, Cyclin D1, Csii Cmv Htert, Pcmv Vsvg Rsv, Pcag Hivgp, And Csii Cmv Egfp, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression plasmids encoding mutant cdk4, cyclin d1, csii-cmv-htert, pcmv-vsvg-rsv, pcag-hivgp, and csii-cmv-egfp/product/BioResource International Inc
Average 90 stars, based on 1 article reviews
expression plasmids encoding mutant cdk4, cyclin d1, csii-cmv-htert, pcmv-vsvg-rsv, pcag-hivgp, and csii-cmv-egfp - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
plasmid encoding viral packaging proteins vsvg  (Promega)
90
Promega plasmid encoding viral packaging proteins vsvg
Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G <t>tsO45</t> released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.
Plasmid Encoding Viral Packaging Proteins Vsvg, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid encoding viral packaging proteins vsvg/product/Promega
Average 90 stars, based on 1 article reviews
plasmid encoding viral packaging proteins vsvg - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G tsO45 released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.

Journal:

Article Title: Rab8 Regulates Basolateral Secretory, But Not Recycling, Traffic at the Recycling Endosome

doi: 10.1091/mbc.E07-09-0902

Figure Lengend Snippet: Ablation of the RE causes mis-sorting of newly synthesized VSV-G. All panels show MDCKT cells (4 d after plating) that have internalized Tfn-HRP into the RE. (A, C, E, G, I, and K) Control cells treated with DAB but not H2O2; (B, D, F, H, J, and L) cells treated with DAB and H2O2. (A and B) Basolaterally applied Alexa-546 Tfn internalized into MDCKT cells for 8 min labels early (small arrow) and recycling (large arrow) endosomes. REs are not visible in RE-ablated cells. (C and D) Basolaterally applied Alexa-546 Tfn internalized into control MDCKT cells for 25 min labels RE (large arrows). Small peripheral early endosomes are visible in RE-ablated cells (small arrows). (E and F) In MDCKT cells labeled for Gp-114, both control and RE-ablated cells are labeled apically. (G and H) In MDCKT cells labeled for p58, both control and RE-ablated cells are labeled basolaterally. (I and J) MDCKT cells expressing VSV-G tsO45 released at the permissive temperature for 2 h after RE ablation. Green arrow in I indicates normal basolateral distribution of VSV-G. Red arrow in J indicates putative apical VSV-G in RE-ablated cells. (K and L) MDCKT cells as in I and J containing cellular GFP-VSV-G (green) and with surface VSV-G labeled by anti-VSV-G ectodomain antibody (red). Red arrow indicates mis-sorted apical VSV-G in ablated cells. Bars, 10 μm.

Article Snippet: We are especially indebted to Agnes Ang, and Ira Mellman (both of Genentech) for providing the dsRFP-Rab8 constructs as well as the YFP-VSV-G tsO45 mutant construct.

Techniques: Synthesized, Control, Labeling, Expressing